The water-soluble CuSO₄ reagent is for performing click reactions in aqueous solution e.g. for experiments with cell culture or sensitive biomolecules that do not tolerate DMSO. In order to create the click reactive Cu(I) species, Na-Ascorbate is needed to reduce Cu(II). Additionally, please make sure using a water-soluble ligand (e.g. THPTA) protecting your biomolecule.

LITERATURE

Functionalization of the surface of electrospun poly(epsilon-caprolactone) mats using zwitterionic poly(carboxybetaine methacrylate) and cell-specific peptide for endothelial progenitor cells capture, Q. Li et al., 2013, Mater. Sci. Eng., Vol. 33(3), p. 1646-1653.

https://doi.org/10.1016/j.msec.2012.12.074

Synthesis of in situ functionalized iron oxide nanoparticles presenting alkyne groups via a continuous process using near-critical and supercritical water, M. D. de Tercero, 2013, J Supercrit Fluids, Vol. 82, p. 83-95.

https://doi.org/10.1016/j.supflu.2013.06.006