NHS-C3-Azide
Bifunctional linker
| Size | Catalog No. | Price |
|---|---|---|
| 5 mg | BCL-014-5 | € 40,00 |
| 10 mg | BCL-014-10 | € 65,00 |
Chemical Properties
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Molecular Formula
C8H10N4O4
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dry, inert gas
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Molecular Weight
226.19 g/mol
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Purity
≥ 95% (NMR)
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Physical State
White to off-white solid
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CAS Number
943858-70-6
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Additional name
gamma-Azidobutyric acid oxysuccinimide ester; γ-Azidobutyric acid NHS; 2,5-dioxopyrrolidin-1-yl 4-azidobutanoate; Azidobutyric NHS ester
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Solubility
DMSO, DMF, DCM
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Preparation/Handling
For a 10 mM solution add 442 μL to 1 mg.
Product Information
NHS-C3-Azide is a hetero-bifunctional reagent that allows to crosslink alkyne (bio-)molecules to compounds bearing primary amines. The NHS ester group will react with primary amines while the azide can undergo click reaction. This linker is often used to introduce azide moieties into amino-oligonucleotides after solid phase synthesis.
LITERATURE
Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking, W. Hou et al., 2019, Scientific Reports, Vol. 9, p. 1307.
https://doi.org/10.1038/s41598-018-37789-9
Integration of a field effect transistor-based aptasensor under a hydrophobic membrane for bioelectronic nose applications, A.E. Kuznetsov et al., 2019, Biosensors and Bioelectronics, Vol. 129, p. 29-35.
https://doi.org/10.1016/j.bios.2019.01.013
Enterotoxigenic Escherichia coli–blood group A interactions intensify diarrheal severity, P. Kumar et al., 2018, J. Clin. Investig., Vol. 128(8), p. 3298-3311.
https://doi.org/10.1172/JCI97659DS1
Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase, M. Andrianova et al., 2018, Sensores, Vol. 18, p. 49.
https://doi.org/10.3390/s18010049
Aptamer based vanillin sensor using an ion-sensitive field-effect transistor, A. Kuznetsov et al., 2018, Microchimica Acta, Vol. 185(1), p. 3.
https://doi.org/10.1007/s00604-017-2586-4
Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence, M. Taskova et al., 2017, Analytical Chemistry, Vol. 89(8), p. 4363–4366.
https://doi.org/10.1021/acs.analchem.7b00646
Gas phase click chemistry via ion/ion reactions, J. Bu et al., 2015, Int J Mass Spectrom, Vol. 309, p. 118-123.
https://doi.org/10.1016/j.ijms.2015.05.010
Two-step labeling of Staphylococcus aureus with Lysostaphin-Azide and DIBO-Alexa using click chemistry, I. Potapova et al., 2013, Journal of Microbiological Methods, Vol. 92(1), p. 90-98.