3′-(O-Propargyl)-GTP

Product Information

Triphosphate for 5′‑End RNA Modification and Click‑Compatible Capping Strategies

3′‑O‑Propargyl‑GTP is a guanosine triphosphate analog carrying a terminal alkyne group at the 3′‑O position of the ribose. This modification enables the introduction of a click‑reactive alkyne at the 5′ end of short or synthetic RNAs meanwhile forming a protective cap structure, using capping enzyme–based approaches that creates a GpppN structure capable of supporting efficient biological activity.

Although 3′‑O‑Propargyl‑GTP can be incorporated enzymatically at the 3′‑end of RNA, its primary application is in 5′‑end engineering, where it is added by a capping enzyme and subsequently methylated. This results in a cap‑mimicking 5′ modification that maintains translational or functional integrity while enabling downstream CuAAC click conjugation with azido‑labeled probes.

Key Features

• Reactive Alkyne at the 5′ Terminus

Provides a clickable handle directly at the RNA 5′ end cap for CuAAC‑based conjugation with azide dyes, ligands, biotin, or functional groups. 

• Cap‑Like Structure for Functional Short RNA Constructs

The enzymatically incorporated and methylated 3′‑O‑Propargyl‑GTP forms a cap‑like 5′ modification, suitable for functionalized small RNAs, synthetic guide RNAs, or engineered RNA reporters. 

• High‑Purity Research Grade

≥ 95% purity ensures reliable performance in RNA modification workflows. 

• Ideal for Short RNA and Synthetic RNA Applications

Supports experimental systems requiring functionalized RNAs independent of full‑length mRNA processes.

Applications

1. 5′‑End Modification of Short or Synthetic RNA

3′‑O‑Propargyl‑GTP can be added enzymatically to mimic a RNA 5′ cap by forming the GpppN structure, introducing an alkyne for precise chemical modification. This is well suited for short RNAs, including functional RNAs, labeled guide RNAs, aptamers, or reporter RNAs.

2. Click‑Chemistry Conjugation at the 5′ Position

The terminal alkyne enables highly selective CuAAC reactions to attach azide‑labeled dyes, affinity tags, peptides, or bioactive molecules. This approach provides a clean and modular way to build custom 5′‑functionalized RNA tools.

3. Construction of Cap‑Like Structures for Functional RNA

Using a capping enzyme, 3′‑O‑Propargyl‑GTP can be incorporated at the RNA 5′ end and then methylated to create a cap‑like structure that maintains compatibility with processes requiring capped or cap‑mimicking RNA ends.

4. Fluorescent or Biochemical RNA Labeling

The clickable 5′ alkyne allows selective introduction of imaging probes or chemical handles, enabling sensitive detection of short RNAs in biochemical assays.