Alkyne-dATP (7-deaza-7-Ethynyl-dATP)
Modified triphosphate for incorporation in PCR reaction
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Alkyne-dATP (7-deaza-7-Ethynyl-dATP) is easily incorporated into DNA by PCR instead of dATP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by click chemistry. We recommend a substitution of 1 – 10% of dATP.
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Molecular Formula
C13H17N4O12P3
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C
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Molecular Weight
514.22 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
10 mM solution in water
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CAS Number
1208963-36-3 (free acid)
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Absorption (max)
λmax = 278 nm
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Additional name
Alkyne-deoxyadenine-5’-triphosphate
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Molecular Formula