Biotin-PEG4-Azide
Biotinylation reagent for labeling by CuAAC
| Size | Catalog No. | Price |
|---|---|---|
| 5 mg | BCFA-234-5 | € 70,00 |
| 10 mg | BCFA-234-10 | € 110,00 |
Chemical Properties
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Molecular Formula
C20H36N6O6S
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
488.6 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
white to light yellow solid to lump
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CAS Number
1309649-57-7
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Solubility
DMSO, DMF
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Preparation/Handling
For a 10 mM solution add 1023 μL to 5 mg.
For a 10 mM solution add 2047 μL to 10 mg.
Product Information
Biotin-PEG4-Azide is a biotin probe that enables the possibility for copper-catalyzed Azide-Alkyne Chemistry (CuAAC) to biotin label alkyne or DBCO modified targets. Once the biotin is attached to a biomolecule, fluorescence microscopy detection can be carried out using the well-known signal enhancement strategy of incubation with a fluorescent streptavidin-conjugate.
The introduction for the hydrophilic PEG4-moity (19 atoms) reduces aggregation and precipitation during the labeling reaction due to an increase in hydrophilicity and also enhances the detection efficiency of the biotin due to reducing sterically hindrance.
LITERATURE
Discovery of an NAD+ analogue with enhanced specificity for PARP1, X. N. Zhang et al., 2022, Chemical Science, Vol. 13(7), p. 1982-1991.
https://doi.org/10.1039/D1SC06256E
Epitopes displayed in a cyclic peptide scaffold bind SARS‐CoV‐2 antibodies, C. Eriksson et al., 2023, ChemBioChem, Vol. 24(15), e202300103.
https://doi.org/10.1002/cbic.202300103
Functionalized scout fragments for site-specific covalent ligand discovery and optimization, V. M. Crowley et al., 2021, ACS Central Science, Vol. 7(4), p. 613-623.