ClickSeq Library Prep Kit Dual Index Primers

Product Information

Primer Index Kit for Illumina-Compatible ClickSeq Dual Indexing (i7 + i5) for 2×12 Reactions

Our Dual Index Primer Kit is specifically designed for use with ClickSeq and Poly(A)-ClickSeq with dual indexing workflows on Illumina sequencing platforms. This kit provides both i7 and i5 indexing primers for dual-indexed library preparation, ideal for users who require enhanced multiplexing capability and improved sample demultiplexing accuracy.

The primers are not included in the standard ClickSeq Kits and must be ordered separately if required.

Key Features

• Fully compatible with all Illumina sequencing instruments.
• Optimized for ClickSeq and Poly(A)-ClickSeq (PACseq) workflows.
• Enables dual indexing for up to 144 libraries (i7 + i5 format).
• Conveniently packaged in a ready-to-use format for streamlined workflow.

Kit Contents (for 2×12 reactions)

1. 12 unique i7 indexed primers.
2. 12 unique i5 indexed primers.

Intended Use

This Dual Index Primer Kit is recommended for users of the following baseclick kits:

• DNA-ClickSeq Library Prep Kit with dual indexing
• RNA-ClickSeq Library Prep Kit with dual indexing
• Poly(A)-ClickSeq Library Prep Kit with dual indexing
• X-ClickSeq Library Prep Kit with dual indexing

It provides cost-effective, sample-specific dual indexing for downstream sequencing based on Illumina i7 and i5 primers, ensuring maximum flexibility and compatibility.

Note:

If you already use your own dual indexing primers, this kit is optional. However, for best performance and compatibility, we recommend using the validated primers provided here.