DNA FISH Kit

How the ClickTech DNA FISH Kit Works

During PCR amplification, the provided Ethynyl Polymerase incorporates a mixture of natural dNTPs and alkyne‑modified EdUTP, generating a “clickable” DNA amplicon. In a subsequent step, the included click reagents covalently attach the fluorescent azide dye of your choice (488, 555, 594, or 647) resulting in intense, stable, and uniformly labeled DNA FISH probes.

• High labeling density: Produces bright, sensitive probes with significantly improved signal‑to‑noise ratio.
• Universal dye options: Four clickable dyes included—green (488), yellow (555), orange (594), red (647)—allowing flexible multicolor FISH assay design.
• The complete workflow in one kit: Contains all reagents for PCR, click labeling, and probe generation.
• Efficient performance: Supports 20 PCR reactions (50 µL each) and 40 labeling reactions for independent FISH experiments.
• High-quality fluorescence characteristics:
  • 488: Ex 496 nm / Em 516 nm
  • 555: Ex 546 nm / Em 579 nm
  • 594: Ex 584 nm / Em 603 nm
  • 647: Ex 643 nm / Em 662 nm
• Storage-friendly: Long shelf life of 12 months when stored at –20 °C.

Applications

1. Cancer Cytogenetics & Oncology Research

FISH is widely used in diagnosing and characterizing cancer, including:

• • Detection of translocations (e.g., BCR‑ABL)
  • Copy number variations (CNVs)
  • Gene amplification (e.g., HER2)

The high sensitivity of ClickTech probes enhances the detection of subtle aberrations in heterogeneous tumor samples.

2. Genetic Syndrome Diagnostics

FISH remains indispensable for identifying chromosomal abnormalities that cause genetic syndromes. Applications include detection of:

• • Microdeletions
  • Trisomies
  • Structural rearrangements

The ClickTech kit’s high labeling density ensures reliable detection even in prenatal cytogenetic samples.

3. Prenatal & Postnatal Screening

ClickTech DNA FISH probes are ideal for rapid screening assays in:

• • Amniocytes
  • Chorionic villi
  • Neonatal blood smear nuclei

The strong signal allows accurate detection even on low‑quality or partially condensed samples.

4. Single‑Cell Genomics & Nuclear Architecture Mapping

Researchers studying 3D genome organization or nuclear compartmentalization can benefit from the kit’s:

• • High probe intensity
  • Reliable locus targeting

This enables elegant visualization of chromosomal territories and long‑range interactions in single nuclei.

5. Detection of Viral Integration or Transgenic Insertions

Bright DNA FISH probes allow precise identification of exogenous sequences in:

• • Viral integration studies
  • CRISPR/Cas9 genome engineering validation
  • Stable transfection screening workflows

6. Chromosomal Spread Analyses in Basic Research

For routine cytogenetic studies involving metaphase spreads, the ClickTech DNA FISH Kit delivers superior clarity, ideal for:

• • Karyotyping research
  • Evolutionary biology
  • Comparative genomics

7. Diagnostic and Research Lab Multiplex Panels

Because the dyes span the green‑to‑far‑red range, the kit supports robust multiplex FISH applications such as:

• • 4‑locus panels
  • Fusion gene co‑localization
  • Multi‑color chromosome painting

LITERATURE

Orthogonal End Labelling of Oligonucleotides through Dual Incorporation of Click-Reactive NTP Analogues. E. Schönegger et al., 2024, ChemBioChem., Vol. 25(1), e202300701.

https://doi.org/10.1002/cbic.202300701

Divergent Synthesis of Ultrabright and Dendritic Xanthenes for Enhanced Click-Chemistry-Based Bioimaging. L. Montiel et al., 2023, Eur. J., Vol. 29(5), e202202633.

https://doi.org/10.1002/chem.202202633

Click Chemistry Enables Rapid Amplification of Full-Length Reverse Transcripts for Long-Read Third Generation Sequencing. E. Schönegger et al., 2022, Bioconjugate Chem., 33(10), p. 1789–1795.

https://doi.org/10.1021/acs.bioconjchem.2c00353

ClampFISH detects individual nucleic acid molecules using click chemistry–based amplification. S. H. Rouhanifard et al., 2019, Biotechnol., Vol 37, p. 84–89.

https://doi.org/10.1038/nbt.4286