DBCO-PEG4-Desthiobiotin
Desthiobiotinylation reagent for labeling by SPAAC
| Size | Catalog No. | Price |
|---|---|---|
| 5 mg | BCFA-233-5 | € 140,00 |
Chemical Properties
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Molecular Formula
C39H53N5O8
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark, inert gas
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Molecular Weight
719.87 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
colorless to slightly yellow oil
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CAS Number
2032788-37-5
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Solubility
DMSO, DMF, MeOH, DCM, THF
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Preparation/Handling
For a 10 mM solution add 695 μL to 5 mg.
For a 10 mM solution add 1389 μL to 10 mg.
Product Information
DBCO-PEG4-Desthiobiotin can be incorporated to biomolecules via strain-promoted Alkyne-Azide Chemistry (SPAAC) which does not need a Cu (I) catalysis or heating. Desthiobiotin binds less tightly to streptavidin than biotin and therefore Desthiobiotin labelled biomolecules can easily be eluted from streptavidin affinity resin by using free biotin.
The introduction of the hydrophilic PEG4-moity reduces aggregation and precipitation during the labeling reaction by increasing the hydrophilicity.
LITERATURE
Quantitative secretome analysis of activated Jurkat cells using click chemistry-based enrichment of secreted glycoproteins, K. E. Witzke et al., 2017, Journal of Proteome Research, Vol. 16(1), p. 137-146.
https://doi.org/10.1021/acs.jproteome.6b00575
Tetracyclines modify translation by targeting key human rRNA substructures, J. D. Mortison et al., 2018, Cell chemical biology, Vol. 25(12), p. 1506-1518.