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Fluorescein Azide (6-FAM)
FITC - Fluorescent dye to label DNA/RNA
- Size
- Catalog No.
- Price
- 1.0 mg
BCFA-001-1
- € 45,00
- 5.0 mg
BCFA-001-5
- € 80,00
- 10 mg
BCFA-001-10
- € 125,00
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6-FAM (Fluorescein)-azide is a marker reagent that allows efficient fluorescent labeling of nucleic acids by click bioconjugation. With identical to almost identical spectral properties to FITC (Fluorescein-5,6-isothiocyanate), ATTO488, Alexa Fluor 488 or DyLight 488 and alike, this compound is well suited for most fluorescent detection equipments.
LITERATURE
P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chemie – Int. Ed. 2008, 47, 8350–8358.
P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, 2008.
W. H. Zhan, H. N. Barnhill, K. Sivakumar, H. Tian, Q. Wang, Tetrahedron Lett. 2005, 46, 1691–1695.
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Molecular Formula
C24H18N4O6
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
458.43 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
yellow to orange solid
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CAS Number
1386385-76-7
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Additional name
6-FAM azide; 6-Carboxyfluoresceine Azide, 4-((3-Azidopropyl)carbamoyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid; N-(3-azidopropyl)-3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carboxamide
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Excitation (max)
50 mM PBS, pH 9: 496 nm
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Emission (max)
50 mM PBS, pH 9: 516 nm
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Ɛ (max)
50 mM PBS, pH 9: 83,000 cm-1M-1
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Solubility
DMSO, DMF, MeOH
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Preparation/Handling
For a 10 mM solution add 218 μL to 1 mg.
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Molecular Formula