We enable nucleic acid labeling bioconjugation
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Fluorescein Azide (6-FAM)

FITC - Fluorescent dye to label DNA/RNA

Currency:  
  • Size
  • Catalog No.
  • Price
  • 1.0 mg
  • BCFA-001-1

  • € 45,00
  • 5.0 mg
  • BCFA-001-5

  • € 80,00
  • 10 mg
  • BCFA-001-10

  • € 125,00
  • 50 mg
  • BCFA-001-50

  • € 320,00
  • 100 mg
  • BCFA-001-100

  • € 500,00
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  • 6-FAM (Fluorescein)-azide is a marker reagent that allows efficient fluorescent labeling of nucleic acids by click bioconjugation. With identical to almost identical spectral properties to FITC (Fluorescein-5,6-isothiocyanate), ATTO488, Alexa Fluor 488 or DyLight 488 and alike, this compound is well suited for most fluorescent detection equipments.

    LITERATURE

    P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chemie – Int. Ed. 2008, 47, 8350–8358.

    P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, 2008.

    W. H. Zhan, H. N. Barnhill, K. Sivakumar, H. Tian, Q. Wang, Tetrahedron Lett. 2005, 46, 1691–1695.

    • Molecular Formula

      C24H18N4O6

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark

    • Molecular Weight

      458.43 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      yellow to orange solid

    • CAS Number

      1386385-76-7

    • Additional name

      6-FAM azide; 6-Carboxyfluoresceine Azide, 4-((3-Azidopropyl)carbamoyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid; N-(3-azidopropyl)-3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carboxamide

    • Absorption (max)

      50 mM PBS, pH 9: 496 nm

    • Emission (max)

      50 mM PBS, pH 9: 516 nm

    • Ɛ (max)

      50 mM PBS, pH 9: 83,000 cm-1M-1

    • Solubility

      DMSO, DMF, MeOH

    • Preparation/Handling

      For a 10 mM solution add 218 μL to 1 mg.

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