PCR Modification Kit
Preparation of fluorescent-labeled DNA products by PCR
| Size | Catalog No. | Price |
|---|---|---|
| Dye 488 | BCK-PCR-MOD-488 | € 500,00 |
| Dye 555 | BCK-PCR-MOD-555 | € 500,00 |
| Dye 594 | BCK-PCR-MOD-594 | € 500,00 |
| Dye 647 | BCK-PCR-MOD-647 | € 500,00 |
-
baseclick’s ClickTech PCR Modification Kit contains all reagents required for generating highly labelled PCR products via an easy-to-handle two-step method based on click chemistry.
The Mixture of unmodified dNTP and alkyne modified- (EdUTP) nucleotides can be used in PCR reactions with the optimized baseclick Ethynyl Polymerase. The resulting PCR products contain alkyne moieties, which react with azido-derivatives of fluorescent dyes, haptnes and other labels in a highly selective fashion under benign click reaction conditions. We offer within this kit click reagents and a clickable dye of your choice (green 488, yellow 555, orange 594 or red 647) obtaining fluorescent labeled PCR products.
-
-
Shelf Life
12 months unopened after receipt
-
Storage Conditions
– 20 °C
-
Physical State
kit system made of different components
-
CAS Number
n.a.
-
Excitation (max)
Dye 488: 496 nm | Dye 555: 546 nm | Dye 594: 584 nm | Dye 647: 643 nm
-
Emission (max)
Dye 488: 516 nm | Dye 555: 579 nm | Dye 594: 603 nm | Dye 647: 662 nm
-
Ɛ (max)
Dye 488: 83.000 cm-1M-1 | Dye 555: 91.000 cm-1M-1 | Dye 594: 110.000 cm-1M-1 | Dye 647: 250.000 cm-1M-1
-
Preparation/Handling
please see user manual of the kit
-
Shelf Life