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Sensitive EdU Cell Proliferation Assay for High-Throughput Screening

ClickTech Sensitive EdU Cell Proliferation Kit for HTS

Size Catalog No. Price
Dye 488 / 2x 96 well plates BCK-EdUPro488HTS2  410,00
Dye 488 / 4x 96 well plates BCK-EdUPro488HTS4  620,00
Clear

Chemical Properties

  • Shelf Life

    12 months unopened after receipt

  • Storage Conditions

    2-8 °C

  • Physical State

    kit system made of different components

  • CAS Number

    n.a.

  • Excitation (max)

    Dye 488: 496 nm

  • Emission (max)

    Dye 488: 516 nm

  • Ɛ (max)

    Dye 488: 83.000 cm-1M-1

  • Preparation/Handling

    please see user manual of the kit

Product Information

High-throughput DNA synthesis detection with minimal cytotoxicity and automation-ready workflow

ClickTech Sensitive EdU Cell Proliferation Kit for HTS is engineered for large-scale screening in 96-well and higher-density formats, delivering precise, reproducible quantification of cell proliferation for drug discovery, toxicity profiling, and compound libraries. The assay uses EdU (5-ethynyl-2′-deoxyuridine) incorporation during DNA synthesis and a bioorthogonal click reaction with picolyl-azide fluorophores, ensuring bright, stable signals and minimal background, ideal for high-content analysis across diverse cell types.

Automation Compatibility

  • Fully compatible with liquid handling robots and automated plate washers
  • Optimized for automated reagent dispensing and high-content screening platforms
  • Seamless integration with LIMS and data analysis pipelines
  • Reagent volumes and incubation times tailored for robotic scheduling
  • Minimal manual intervention, ideal for unattended batch processing

Key Benefits

  • Exceptional Sensitivity with low background: Optimized labeling chemistry and picolyl‑azide fluorophore deliver high signal‑to‑noise even in sparse wells (≈ ≥ 500 cells/well) and low‑proliferation conditions.
  • Non-Radioactive & Safer: Eliminates hazards of [³H]-thymidine and BrdU; no harsh treatments or specialized equipment.
  • Fast, Simplified Workflow: Complete the assay in under 2 hours, no DNA denaturation or antibody steps; fully automation-ready.
  • Low Copper, High Viability: The click step is formulated with reduced copper versus conventional EdU protocols, supporting cell health during longer incubations and preserving compatibility with downstream readouts.
  • Automation-Ready: Assay timings, wash steps, and reagent additions are tuned for liquid handlers, automated plate washers, and unattended batch processing, with consistent performance across plates, days, and operators.
  • Scalable & Reproducible: Bioorthogonal chemistry supports thousands of wells with consistent performance across plates and runs.

Recommended workflow (plateready)

  1. Cell preparation & EdU pulse: Seed cells in black, clear‑bottom plates. Pulse with EdU during the intended window (e.g., 15–120 min). Short pulses capture S‑phase entry; longer pulses give cumulative labeling for slow cyclers.
  2. Fix & permeabilize: Apply standard aldehyde fixation followed by mild permeabilization to retain morphology and antigenicity for imaging or multiplex staining.
  3. Click detection: Add the picolyl‑azide 488 dye cocktail. Incubate under gentle conditions, wash.
  4. Optional multiplex: Introduce viability dyes, phenotypic markers, or nuclear stains (for HCS segmentation) as needed.
  5. Readout:
  • Plate reader: bottom read with 488‑compatible excitation and 516 nm emission filter.
  • HCS/Imaging: use the green channel; quantify EdU‑positive nuclei or integrated intensity per cell.
ClickTech Sensitive EdU Cell Proliferation Kit for HTS

Plate normalization tip: include row/column controls to support ratio‑based normalization and drift correction; monitor Z′‑factor and well‑to‑well CV before production runs.

Applications

  • High-throughput proliferation and cell-cycle screening
  • Genotoxicity and mechanism-of-action studies
  • Anticancer compound profiling and SAR workflows
  • Stem cell proliferation and differentiation monitoring
  • Orthogonal readouts with viability/apoptosis markers or HCS imaging

 

LITERATURE

EdU Incorporation To Assess Cell Proliferation and Drug Susceptibility in Naegleria fowleri. 2021, Antimicrob Agents Chemother.

https://doi.org/1128/AAC.00017-21

Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop. Nature Communications. 2022.

https://doi.org/10.1038/s41467-022-34536-7

Efficient Tandem Copper‐Catalyzed Click Synthesis of Multisugar‐Modified Oligonucleotides. Angewandte Chemie International Edition, e202405161.

https://doi.org/10.1002/anie.202405161

FAQ

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