L-lysine hydrochloride in research & pharma
L-lysine hydrochloride is an essential amino acid crucial for protein synthesis, tissue repair and enzyme production. L-lysine hydrochloride is water-soluble, making it highly effective in a wide range of biochemical applications. In biochemistry, L-lysine HCl salt is used extensively in cell culture studies, protein synthesis and genetic research. Its solubility allows for better control in experiments, ensuring reproducible and accurate results. This compound also contributes to pharmaceutical research, especially in drug development, as well as the production of protein-based medications, vaccines, and treatments for lysine deficiencies. In genetic research, it is vital for nucleic acid modification to the nucleobase and click chemistry applications. It is used in the preparation of oligonucleotides for genetic studies, such as DNA and RNA sequencing and gene expression analysis. Through to its ability to bind to the phosphate backbone of nucleic acids it is used in DNA and RNA sequencing. For better following of the amino acids modified variants as 6-Azido-L-lysine hydrochloride are used as replacement. This chemical modification enables the possibility to label the amino acid via click chemistry with alkynes or DBCO containing reporter molecules as fluorophores or dyes.
Overall, L-lysine hydrochloride is a critical tool in both biochemistry and pharmaceutical industries, offering stability, solubility and versatility for scientific research and drug development.
What is L-lysine hydrochloride?
L-lysine hydrochloride is the hydrochloride salt form of L-lysine, an amino acid that the body cannot produce on its own and must be obtained through diet or supplementation. L-lysine is necessary for the synthesis of proteins, enzymes, and hormones and plays a key part in tissue repair and growth. The hydrochloride form of L-lysine is created by combining L-lysine with hydrochloric acid, which enhances the compound’s solubility, manageability and stability, making it more suitable for a range of scientific applications.
The chemical structure consists of the protonated amino acid L-lysine with a Cl– as counterion for the positively charged amino functionality. This makes the compound highly soluble in water, unlike its free base form, L-lysine, which is only sparingly soluble. The production of the hydrochloric acid salt helps to stabilize the compound and allows it to dissolve more easily in aqueous solutions, which is necessary in laboratory settings where precise concentrations and reproducibility are necessary. It also changes the substances from a hardly weighable oil to a easy to handle powder.
L-lysine hydrochloride is typically derived from natural sources such as proteins found in animal- and plant-based foods. It can also be synthetically produced through fermentation processes, where specific bacteria are used to produce amino acids. This makes it widely available for research and industrial purposes.
In summary, L-lysine hydrochloride is a compound for scientific research due to its improved solubility, stability, and availability compared to pure L-lysine. These properties make it invaluable in a wide range of biochemical and pharmaceutical applications, supporting research in areas like cell culture, protein synthesis and drug development. For better following of L-Lysine during biological modifications, there are modified versions available, which can be postsynthetically reacted to label molecules. A common modification is an azide which allows for click chemistry modification by copper catalyzed alkyne azide cycloaddition (CuAAC) or strain promoted alkyne azide cycloaddition (SPAAC).
Applications in biochemistry & research
L-lysine hydrochloride plays a significant role in various biochemical and molecular biology applications, making it an indispensable reagent for researchers in these fields. Its unique properties, such as water solubility and stability, make it valuable in experiments that require precise control over chemical conditions.
One of the primary applications is cell culture. It is commonly used as a supplement in culture media to support the growth and proliferation of mammalian cells. Since L-lysine is an amino acid that these cells cannot synthesize on their own, adding L-lysine hydrochloride ensures that the cells have a constant and sufficient supply of this critical nutrient, which is necessary for protein synthesis and overall cellular function.
In molecular biology experiments, L-lysine hydrochloride is utilized in a variety of ways. It serves as a key ingredient in buffer solutions and reaction mixtures in processes such as PCR (Polymerase Chain Reaction), which supports the stability of the DNA template and facilitates the synthesis of nucleic acids. Additionally, its azide containing version 6-Azido-L-lysine hydrochloride is involved in experiments that require the modification of proteins and nucleic acids, contributing to the efficiency of these procedures.
Pharmaceutical research
L-Lysine is an essential amino acid with widespread applications in human health and drug development:
- Health & Biological Function:
– Supports protein and collagen synthesis, calcium absorption, and immune response
– Crucial for wound healing, bone strength, and overall metabolism
- Therapeutic Potentia:
– Shows antiviral activity (especially against HSV)
– Aids in osteoporosis, stress, and possibly cancer therapies
– Studied for insulin support and pain relief
- Formulation & Drug Delivery
– Improves solubility, permeability, and bioavailability of drug compounds
– Acts as a buffer, stabilizer, and lyoprotectant in protein-based drugs
- Research & Engineering Applications
– Used for chemical conjugation (PEGylation, antibody-drug conjugates)
– Enhances cell adhesion in culture and neurotransmitter signaling in brain studies
Genetic research
L-lysine hydrochloride is also highly significant in genetic research, where it is involved in various applications. The compound is a key player in the synthesis of modified nucleotides, which are pivotal for genetic studies such as DNA and RNA sequencing, gene expression analysis and genetic engineering. These modified nucleotides help researchers analyze gene function, study mutations and investigate how genetic material influences cellular processes.
Additionally, it is a vital component in genetic assays used for detecting and analyzing genetic sequences. By contributing to the preparation of primers, probes and other reagents in these assays, it ensures that researchers can obtain accurate and reliable data for their genetic studies.
One of the most exciting applications of L-lysine hydrochloride and its modifications in genetic research is its involvement in click chemistry. Click chemistry is a powerful tool to modify and tag nucleic acids, proteins and other biomolecules for further analysis. It is often used in click chemistry reactions to create conjugates that enable researchers to track and study biomolecular interactions in greater detail.
Learn more about Click Chemistry here: Click Chemistry
In conclusion, L-lysine hydrochloride is indispensable in both genetic and pharmaceutical research. Its ability to support nucleic acid modification, enable advanced genetic assays and contribute to click chemistry processes makes it a key tool for researchers working on genetic engineering, drug development and biopharmaceutical production. baseclick’s innovations in click chemistry further enhance its application, driving advancements in both fields.
Benefits & advantages of L-lysine hydrochloride
L-lysine hydrochloride is efficient in chemical reactions. Its water solubility and stability enable it to perform effectively in a wide range of chemical processes. Whether it’s used in the synthesis of proteins, modification of nucleic acids, or gene therapy research, L-lysine hydrochloride reacts quickly and consistently, making it a reliable reagent for experiments that require precision and reliability.
Why choose L-lysine hydrochloride
L-lysine hydrochloride is chosen for its high water solubility, stability, and enhanced bioavailability, making it ideal for nutritional supplements and pharmaceutical formulations. It supports protein synthesis, calcium absorption, and immune function especially important since the body can’t produce lysine on its own. For advanced research, 6-Azido-L-lysine HCl from baseclick offers a powerful tool for protein labeling and bioconjugation. Its azide group enables click chemistry, allowing precise attachment of molecules like PEGs, antibodies, or fluorescent tags perfect for studying protein synthesis and cellular processes.
By choosing baseclick, researchers can be confident that they are using top-tier products that contributes to the success of their projects. The company’s dedication to quality and customer satisfaction makes it the preferred choice for professionals looking for dependable and effective biochemical reagents. Whether for cell culture, protein synthesis, or genetic research, baseclick’s high-quality 6-azido-L-lysine hydrochloride helps drive innovation and ensures the accuracy of experimental results.
