We enable nucleic acid labeling bioconjugation
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5-Ethynyl-dUTP

Modified Triphosphate for chemo-enzymatic labeling of long oligonucleotides

Currency:  
  • Size
  • Catalog No.
  • Price
  • 0.5 µmol
  • BCT-08-S

  • € 75,00
  • 2.5 µmol
  • BCT-08-M

  • € 120,00
  • 5.0 µmol
  • BCT-08-L

  • € 240,00
Clear
  • 5′-Ethynyl-dNTP can be easily incorporated into DNA by PCR using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified (we recommend a modification of 1-10% of the corresponding natural base only) PCR fragment is now ready for further functionalization with a.g. dyes, biomolecules, oligos, etc.

    LITERATURE

    J. Gierlich, K. Gutsmiedl, P. M. E. Gramlich, A. Schmidt, G. A. Burley, T. Carell, Chem. – A Eur. J. 2007, 13, 9486–9494.

    G. A. Burley, J. Gierlich, M. R. Mofid, H. Nir, S. Tal, Y. Eichen, T. Carell, J. Am. Chem. Soc. 2006, 128, 1398–1399.

    • Molecular Formula

      C11H15N2O14P3

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C

    • Molecular Weight

      492.16 g/mol

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      100 mM clear colorless solution in water (pH 7,5)

    • CAS Number

      1004955-96-7

    • Additional name

      EdUTP; 5-Ethynyl-2’-deoxyuridine-5’-triphosphate

    • Absorption (max)

      λmax = 288 nm

    • Ɛ (max)

      12,000 cm-1M-1

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