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Amino-hexynyl-pApG

Capping Initiator for 5′-end mRNA labeling via NHS Ester Chemistry

Size Catalog No. Price
1 µmol BCT-40-S  320,00
5 µmol BCT-40-L  960,00
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  • Product Overview

    Amino-hexynyl-pApG is a chemically synthesized capping initiator designed for site-specific 5′-end modification of mRNA during in vitro transcription (IVT). Upon incorporation at the 5′-end, this dinucleotide analog introduces a primary amine functional group, enabling bio-orthogonal labeling of capped mRNA through NHS ester-based conjugation.

    While it does not serve as a traditional cap structure that promotes ribosome recruitment, Amino-hexynyl-pApG enhances mRNA stability and maintains a level of translation efficiency that is superior to uncapped RNA. It also functions as a versatile reporter, supporting a wide range of chemical modifications at the 5′-terminus.

     

    Addressing a key limitation in mRNA labeling

    Conventional mRNA modification strategies are predominantly focused on the 3′-end, where enzymatic addition of functional groups enables bioconjugation. However, targeted 5′-end modifications have remained limited due to the complexity of preserving cap structure and functionality.

    Amino-hexynyl-pApG offers a direct and efficient solution for 5′-end functionalization of enzymatically capped mRNA without disrupting the transcript’s integrity, allowing for new experimental and therapeutic capabilities.

     

    Benefits & Applications

    • Introduces a primary amine at the mRNA 5′-end
    • Enables site-specific labeling via NHS ester conjugation
    • Compatible with enzymatically capped mRNA
    • Useful in fluorescent tagging, surface immobilization, and targeted delivery
    • Expands options for dual-end labeling (5′ and 3′)
    • Supports development of RNA therapeutics, including cell-specific targeting

     

    Use Cases

    • Fluorescent and affinity labeling of synthetic mRNA for imaging and tracking
    • Conjugation of peptides, ligands, nanoparticles, or biotin via NHS esters
    • Engineering targeted mRNA constructs for delivery or replacement therapies
    • Investigating mRNA structure–function relationships or RNA–protein interactions

     

    Summary

    Amino-hexynyl-pApG provides researchers with a powerful and flexible tool for site-selective modification of the mRNA 5′-end. By enabling primary amine-mediated conjugation through NHS ester chemistry, it unlocks new potential in mRNA labeling, imaging, and therapeutic development — complementing traditional 3′-modification strategies and advancing the field of synthetic RNA engineering.

    • Molecular Formula

      C26H39N11O14P2 (free acid)

    • Shelf Life

      12 months unopened after receipt

    • Storage Conditions

      -20 °C, dark, dry

    • Molecular Weight

      791.60 g/mol (free acid)

    • Purity

      ≥ 95% (HPLC)

    • Physical State

      colorless solution (10 mM)

    • CAS Number

      n.a.

    • Absorption (max)

      λmax = 256 nm

    • Ɛ (max)

      25.050 cm-1M-1

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