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AZDye 488 Azide Plus

Improved 488 dye to label with reduced copper concentrations

Size Catalog No. Price
1 mg BCFA-236-1  200,00
5 mg BCFA-236-5  650,00
Clear

Chemical Properties

  • Molecular Formula

    C29H30N10O10S2

  • Shelf Life

    12 months unopened after receipt

  • Storage Conditions

    -20 °C, dark

  • Molecular Weight

    754.75 g/mol

  • Purity

    ≥ 95% (HPLC)

  • Physical State

    orange to red solid

  • CAS Number

    n.a.

  • Excitation (max)

    495 nm

  • Emission (max)

    517 nm

  • Ɛ (max)

    73.000 cm-1M-1

  • Solubility

    H2O, DMSO, DMF

  • Preparation/Handling

    For a 10 mM solution add 132 μL to 1 mg.
    For a 10 mM solution add 662 μL to 5 mg.

Product Information

Enhanced Fluorescent Probe for Bioorthogonal Labeling via Click Chemistry

AZDye™ 488 Azide Plus is a green-fluorescent, water-soluble azide probe optimized for copper-catalyzed and copper-free click chemistry. It enables rapid, efficient, and low-background labeling of alkyne-functionalized biomolecules in live-cell imaging, flow cytometry, and biochemical assays.

AZDye™ 488 Azide Plus is a next-generation fluorescent azide probe designed for high-performance bioconjugation. It reacts with terminal alkynes via Copper(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) and with strained cyclooctynes via copper-free click chemistry, forming stable triazole linkages under mild conditions.

CuAAC reaction between AZDye 488 Azide plus and alkyne functionalized molecule

CuAAC reaction between AZDye 488 Azide plus and alkyne functionalized molecule

This variant introduces a copper-chelating system that enhances CuAAC efficiency by forming active copper complexes. As a result, AZDye™ 488 Azide Plus functions both as a reactant and as a catalytic enhancer, reducing copper requirements and improving labeling performance.

Advantages of AZDye™ 488 Azide Plus

  • Reduced Copper Requirements: The built-in chelating system lowers the need for external copper, minimizing cytotoxicity in sensitive biological systems.
  • Accelerated Reaction Kinetics: Enables faster and more complete conjugation with alkyne-functionalized targets.
  • High Sensitivity: Ideal for detecting low-abundance biomolecules in complex samples.
  • Superior Signal-to-Noise Ratio: Delivers sharp fluorescence with minimal background interference.
  • Copper-Free Compatibility: Compatible with copper-sensitive biomolecules and workflows, preserving enzyme activity and cell viability.

Applications of AZDye™ 488 Azide Plus

  • Live-Cell and Fixed Sample Labeling
    Suitable for direct imaging in cellular systems where long-lasting fluorescence and minimal toxicity are essential.
  • Flow Cytometry and High-Content Screening
    Excitation at 488 nm provides strong signal clarity for quantitative analysis.
  • Biochemical Assays
    High quantum yield and low non-specific binding improve assay precision.
  • Aqueous Labeling
    Water-soluble formulation supports efficient conjugation in aqueous buffers without organic solvents.

To complement AZDye™ 488 Azide Plus, baseclick now offers DBCO-AF488, a copper-free fluorophore for bioorthogonal labeling. DBCO-AF488 enables efficient conjugation to azide-functionalized biomolecules without the need for copper catalysts, making it ideal for live-cell applications and workflows sensitive to metal ions.

 

LITERATURE

Engineering of cell-surface receptors for analysis of receptor internalization and detection of receptor-specific glycosylation, C. H. Lee et al., 2024, Chem. Sci., Vol. 15(2), p. 555-565.

https://doi.org/10.1039/D3SC05054H

In-depth immunometabolic profiling by measuring single-cell protein translation inhibition via bioorthogonal noncanonical amino acid tagging (SCENITH-BONCAT), F. Vrieling et al., 2023, bioRxiv, 2023-08.

https://doi.org/10.1101/2023.08.03.551800

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