AZDye 647 Azide Plus
Improved 647 dye to label with reduced copper concentrations
| Size | Catalog No. | Price |
|---|---|---|
| 1 mg | BCFA-242-1 | € 200,00 |
| 5 mg | BCFA-242-5 | € 650,00 |
Chemical Properties
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Molecular Formula
C46H64N10O13S4
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
1093.3 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
blue solid
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CAS Number
n.a.
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Excitation (max)
648 nm
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Emission (max)
671 nm
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Ɛ (max)
270.000 cm-1M-1
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Solubility
H2O, DMSO, DMF
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Preparation/Handling
For a 10 mM solution add 91.4 μL to 1 mg.
For a 10 mM solution add 457 μL to 5 mg.
Product Information
Far-Red Fluorescent Dye for High-Efficiency Click Chemistry
AZDye 647 Azide Plus is a premium far-red fluorescent dye engineered for advanced copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions. Featuring a proprietary copper-chelating molecular structure, it forms a highly active copper complex that functions as both reactant and catalyst, enabling efficient click chemistry even at low copper concentrations. This makes it ideal for labeling copper-sensitive biomolecules, including enzymes and proteins.
Key Features & Benefits
- Spectral Compatibility: Excitation at ~648 nm and emission at ~671 nm comparable to Alexa Fluor® 647, Cy5®, and CF® 647, ensures seamless integration with standard fluorescence detection systems.
- High Photostability & Bright Signal: Delivers robust fluorescence and long-term imaging performance, enabling detection of low-abundance targets with minimal signal loss.
- Water-Soluble & pH-Stable: Fully soluble in aqueous buffers and stable across a wide pH range (pH 4–10), supporting diverse biological and chemical workflows.
- Low Autofluorescence: Reduces background noise in biological samples, enhancing signal-to-noise ratio and image clarity.
- Enhanced Click Reaction Kinetics: The integrated copper-chelating system enables instantaneous reaction with alkynes, even under diluted conditions—boosting biocompatibility and sensitivity for demanding applications.
Applications
- Fluorescent labeling of proteins, nucleic acids, and other biomolecules
- Live-cell imaging and fixed-cell microscopy
- Bioorthogonal chemistry in sensitive environments
- High-throughput screening and diagnostic assays
Complementary Products from baseclick
- Explore baseclick’s full range of azide-functionalized dyes for click chemistry:
- Eterneon-Red 645 Azide – A cost-effective alternative for standard labeling needs without enhanced kinetics.
- Eterneon-Red 645-Picolyl-Azide – Includes a click-enhancing linker for improved reaction speed and efficiency.
LITERATURE
Optimized incorporation of alkynyl fatty acid analogs for the detection of fatty acylated proteins using click chemistry, L. M. Q. Liao et al., 2021, JoVE (Journal of Visualized Experiments), Vol. 170, e62107.
Acute Oxidative Stress Can Paradoxically Suppress Human NRF2 Protein Synthesis by Inhibiting Global Protein Translation, K. M. Pensabene et al., 2023, Antioxidants, Vol. 12(9), 1735.
https://doi.org/10.3390/antiox12091735
FAQ
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What is AZDye 647 Azide Plus used for?
AZDye 647 Azide Plus is a far-red fluorescent dye designed for high-efficiency click chemistry labeling, especially in copper-sensitive biological systems. It’s ideal for imaging, protein tagging, and bioorthogonal reactions.
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How does AZDye 647 Azide Plus differ from standard azide dyes?
Unlike standard azide dyes, AZDye 647 Azide Plus features a built-in copper-chelating system that enhances reaction speed and efficiency, even at low copper concentrations. This makes it highly suitable for sensitive applications.
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Is AZDye 647 Azide Plus compatible with Alexa Fluor 647 or Cy5?
Yes. AZDye 647 Azide Plus has excitation/emission maxima (~648/671 nm) that are nearly identical to Alexa Fluor® 647, Cy5®, and CF® 647, ensuring compatibility with most fluorescence detection systems.
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Can AZDye 647 Azide Plus be used in live-cell imaging?
Yes. Its high photostability, low autofluorescence, and biocompatibility make it suitable for both live-cell and fixed-cell imaging workflows.
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What buffer conditions are optimal for AZDye 647 Azide Plus?
The dye is water-soluble and stable across a wide pH range (4–10), making it adaptable to various buffer systems and experimental setups.
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Does AZDye 647 Azide Plus require high copper concentrations?
No. Thanks to its integrated copper-chelating structure, it performs efficiently at low copper levels, reducing toxicity and improving compatibility with sensitive biomolecules.
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What are some complementary products for AZDye 647 Azide Plus?
baseclick offers:
- Eterneon-Red 645 Azide – A budget-friendly alternative for standard labeling.
- Eterneon-Red 645-Picolyl-Azide – Includes a click-enhancing linker for faster reactions.
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Is AZDye 647 Azide Plus suitable for detecting low-abundance targets?
Absolutely. Its strong fluorescence signal and enhanced reaction kinetics make it ideal for detecting low-abundance proteins, nucleic acids, and other biomolecules.
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What makes AZDye 647 Azide Plus photostable?
Its molecular design resists photobleaching, allowing for long-term imaging without significant signal loss.
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Where can I buy AZDye 647 Azide Plus?
AZDye 647 Azide Plus is available through baseclick’s webshop and authorized distributors. Contact baseclick for bulk orders or technical support.