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DBCO-AF647

Dye to label by SPAAC in copper-free environment

Size Catalog No. Price
1 mg BCFA-243-1  200,00
5 mg BCFA-243-5  650,00
Clear

Chemical Properties

  • Molecular Formula

    C55H62N4O14S4

  • Shelf Life

    12 months unopened after receipt

  • Storage Conditions

    -20 °C, dark

  • Molecular Weight

    1117.33 g/mol

  • Purity

    ≥ 95% (HPLC)

  • Physical State

    blue solid

  • CAS Number

    n.a.

  • Excitation (max)

    648 nm

  • Emission (max)

    671 nm

  • Ɛ (max)

    270.000 cm-1M-1

  • Solubility

    H2O, DMSO, DMF

  • Preparation/Handling

    For a 10 mM solution add 89.5 μL to 1 mg.
    For a 10 mM solution add 447.5 μL to 5 mg.

Product Information

Far-Red Dye for Copper-Free Click Chemistry

DBCO-AF647 is a high-performance far-red fluorescent dye designed for bioorthogonal labeling via strain-promoted azide–alkyne cycloaddition (SPAAC). This copper-free click reaction eliminates the need for Cu(I) catalysis or heat, making it ideal for live-cell imaging and reactions in sensitive biological systems.

 

Why Choose DBCO-AF647?

  • Rapid, selective conjugation: Highly reactive DBCO group forms stable triazole linkages with azides.
  • Multiplex compatibility: Does not cross-react with tetrazines, enabling use alongside TCO-based systems.
  • Bright far-red signal: Excitation 648 nm / Emission 671 nm for deep-tissue imaging and minimal background.
  • Water-soluble & pH-stable: Maintains integrity across pH 4–10.
  • Low autofluorescence: Long-wavelength emission reduces sample interference.
  • High photostability: Supports long-term, high-resolution imaging.
  • Platform-ready: Spectrally like Cy5, Alexa Fluor® 647, CF® 647, drop-in compatibility with existing setups.
Strain Promoted Azide Alkyne Cycloaddition of DBCO-AF647

Applications of DBCO-AF647

  • Fluorescence microscopy: Live-cell, fixed samples, and whole-organism imaging.
  • Flow cytometry: Compatible with standard red laser lines for multiparametric analysis.
  • Bioorthogonal labeling: Copper-free tagging of proteins, peptides, antibodies, and azide-modified oligonucleotides.
  • Bioconjugation: Amine-reactive functionality for covalent attachment to biomolecules.
  • Tissue imaging: Clear signal even in high-autofluorescence environments.

Technical Specs

  • Excitation / Emission: 648 nm / 671 nm
  • Solubility: Water-soluble
  • pH Stability: 4–10
  • Photostability: High
  • Format: Reactive dye with DBCO group

Complementary Products:

baseclick also offers a comprehensive portfolio of azide-containing dyes for click chemistry applications, including:

 

LITERATURE

Chemoselective Bioconjugation of Amyloidogenic Protein Antigens to PEGylated Microspheres Enables Detection of α-Synuclein Autoantibodies in Human Plasma, P. Ge et al., 2022, Bioconjugate Chemistry, Vol. 33(2), p. 301-310.

https://doi.org/10.1021/acs.bioconjchem.1c00530

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