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DBCO-AF647

Dye to label by SPAAC in copper-free environment

Size Catalog No. Price
1 mg BCFA-243-1  200,00
5 mg BCFA-243-5  650,00
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Chemical Properties

  • Molecular Formula

    C55H62N4O14S4

  • Shelf Life

    12 months unopened after receipt

  • Storage Conditions

    -20 °C, dark

  • Molecular Weight

    1117.33 g/mol

  • Purity

    ≥ 95% (HPLC)

  • Physical State

    blue solid

  • CAS Number

    n.a.

  • Excitation (max)

    648 nm

  • Emission (max)

    671 nm

  • Ɛ (max)

    270.000 cm-1M-1

  • Solubility

    H2O, DMSO, DMF

  • Preparation/Handling

    For a 10 mM solution add 89.5 μL to 1 mg.
    For a 10 mM solution add 447.5 μL to 5 mg.

Product Information

This special red fluorescent dye DBCO-AF647 enables the possibility for copper-free click chemistry which is mostly used in reaction where cytotoxic copper catalyst is not an option. The click reaction takes place via strain-promoted Alkyne-Azide Chemistry (SPAAC) which does not need a Cu (I) catalysis or heating. Once attached to a biomolecule, fluorescence microscopy detection can be carried out just like with Cy5, or any other alternative dye.

 

LITERATURE

Chemoselective Bioconjugation of Amyloidogenic Protein Antigens to PEGylated Microspheres Enables Detection of α-Synuclein Autoantibodies in Human Plasma, P. Ge et al., 2022, Bioconjugate Chemistry, Vol. 33(2), p. 301-310.

https://doi.org/10.1021/acs.bioconjchem.1c00530

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