5-Ethynyl uridine (5-EU)
Clickable EU allows detection of de novo RNA
| Size | Catalog No. | Price |
|---|---|---|
| 10 mg | BCN-003-10 | € 65,00 |
| 100 mg | BCN-003-100 | € 400,00 |
Chemical Properties
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Molecular Formula
C11H12N2O6
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dry, inert gas
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Molecular Weight
268.22 g/mol
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Purity
≥ 98% (HPLC)
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Physical State
white to light brown to light green powder
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CAS Number
69075-42-9
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Additional name
EU, 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione
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Solubility
water, aqueous buffer, DMSO, ethanol
Product Information
5-Ethynyl uridine (5-EU) is a modified nucleoside that can be used for labeling of nascent RNA inside cells. After feeding EU, it can react with azides. Our own experiments indicate that it will be first integrated in de novo synthesized RNA, but we have also observed incorporation into DNA after some incubation time. This is most likely via conversion of the EU ribonucleoside into EdU aided by intracellular ribonucleotide reductases. Research from several groups demonstrates successful EU cellular labeling for divers applications.
LITERATURE
Exploring RNA transcription and turnover in vivo by using click chemistry, C. Y. Jao et al., 2008, Proc. Natl. Acad. Sci. U. S. A., Vol. 105, p. 15779–15784.
https://doi.org/10.1073/pnas.0808480105
Capturing the interactome of newly transcribed RNA, X. Bao et al., 2018, Nature Methods, Vol. 15, p. 213-220.
https://doi.org/10.1038/nmeth.4595
Visualization of the Nucleolus Using Ethynyl Uridine, M. Dvořáčková et al., 2018, Frontiers in Plant Science, Vol. 9(177).