DBCO-AF488
Dye to label by SPAAC in copper-free environment
| Size | Catalog No. | Price |
|---|---|---|
| 1 mg | BCFA-237-1 | € 200,00 |
| 5 mg | BCFA-237-5 | € 650,00 |
Chemical Properties
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Molecular Formula
C39H28N4O11S2
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
792.8 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
orange solid
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CAS Number
423960-92-1
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Excitation (max)
495 nm
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Emission (max)
517 nm
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Ɛ (max)
73.000 cm-1M-1
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Solubility
H2O, DMSO, DMF
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Preparation/Handling
For a 10 mM solution add 126 μL to 1 mg.
For a 10 mM solution add 631 μL to 5 mg.
Product Information
This green-fluorescent DBCO-AF488 dye enables the possibility for copper-free click chemistry which is mostly used in reaction where cytotoxic copper catalyst is not an option. The click reaction takes place via strain-promoted Alkyne-Azide Chemistry (SPAAC) which does not need a Cu (I) catalysis or heating. Once clicked to a biomolecule fluorescence detection is identical to FAM, FITC, or any other alternative dye.
LITERATURE
A metabolic labeling way to in situ fabricate bacterial FRET Platform for innate immune defence molecule, Z. Zhang et al., 2022, Sensors and Actuators B: Chemical, Vol. 350, 130913.
https://doi.org/10.1016/j.snb.2021.130913
Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes, H. M. Micek et al., 2023, APL Bioengineering, Vol. 7(1), 016111.
https://doi.org/10.1063/5.0132254
Investigating Peptidoglycan Recycling Pathways in Tannerella forsythia with N-Acetylmuramic Acid Bioorthogonal Probes, K. A. Wodzanowski et al., 2022, ACS Infect. Dis., Vol. 8(9), p. 1831–1838.