Eterneon-Red 645 Azide
Special Fluorescent Short Stoke Shift Dye to label DNA/RNA
| Size | Catalog No. | Price |
|---|---|---|
| 1 mg | BCFA-201-1 | € 120,00 |
| 5 mg | BCFA-201-5 | € 450,00 |
Chemical Properties
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Molecular Formula
C30H35N5O6S2
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Shelf Life
12 months unopened after receipt
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Storage Conditions
-20 °C, dark
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Molecular Weight
625.76 g/mol
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Purity
≥ 95% (HPLC)
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Physical State
blue to metallic solid
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CAS Number
n.a.
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Additional name
Disulfonate pentamethine cyanine azide
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Excitation (max)
643 nm
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Emission (max)
662 nm
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Ɛ (max)
250,000 cm-1M-1
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Solubility
H2O, DMSO, DMF, MeOH, MeCN
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Preparation/Handling
For a 10 mM solution add 159 μL to 1 mg.
For a 10 mM solution add 799 μL to 5 mg.
Product Information
High-Performance Fluorescent Dye for Click Chemistry
Eterneon-Red 645 Azide (Disulfonate pentamethine cyanine azide) is a water-soluble, red-emitting fluorescent dye designed for bioorthogonal labeling via click chemistry. As a powerful alternative to Cy5, it enables efficient conjugation with alkynylated biomolecules in aqueous environments, making it ideal for bioconjugation, cell proliferation assays, and fluorescence-based detection in molecular biology and diagnostics.
Key Features
- High Sensitivity: Enables precise quantification of DNA synthesis and cell proliferation.
- Bright Fluorescence: Excitation at 643 nm and emission at 662 nm for sharp signal detection.
- Click Chemistry Compatibility: Reacts efficiently via CuAAC with terminal alkynes.
- Water Solubility: Facilitates use in biological buffers and aqueous systems.
- Excellent Stability: Maintains integrity across ionic and pH variations.
- Non-Toxic: Preserves cell viability during in vitro and in vivo applications.
- Versatile Use: Compatible with flow cytometry, fluorescence microscopy, and hybridization-based assays.
Reaction scheme of the complexation of copper by the azide plus linker structure.
Applications
- EdU Cell Proliferation Assays: Detects newly synthesized DNA with high accuracy.
- Click Reaction Efficiency Studies: Evaluates yield, oligo stability, and ionic effects.
- RNA/DNA Labeling: Suitable for ISH/FISH, blots, and multiplexed probe generation.
- Live Cell Imaging: Enables dynamic tracking of biomolecules in real time.
In addition to Eterneon-Red 645 Azide, baseclick also offers Eterneon-Red 645-Picolyl Azide and AZDye 647 Azide Plus, advanced fluorescent dyes featuring picolyl-azide and triazol-azide linker structures, respectively, that enhance click reaction kinetics. By complexing with copper, they ensure efficient conjugation while allowing lower copper concentrations, making them ideal for labeling sensitive biomolecules. These improved designs enable high reaction efficiency, even in challenging environments, and support fluorescence microscopy detection with exceptional stability.
LITERATURE
Efficient DNA Click Reaction Replaces Enzymatic Ligation, M. Kollaschinski et al., 2019, Bioconjugate Chemistry, Vol. 31(3), p. 507–512.
https://doi.org/10.1021/acs.bioconjchem.9b00805
Epigenetic mechanisms controlling human leukemia stem cells and therapy resistance, S. Takao et al., 2022, bioRxiv.
https://doi.org/10.1101/2022.09.22.509005
ARID1A Regulates Transcription and the Epigenetic Landscape via POLE and DMAP1 While ARID1A Deficiency or Pharmacological Inhibition Sensitizes Germ Cell Tumor Cells to ATR Inhibition, L. Kurz et al., 2020, Cancers, Vol. 12(4), p. 905.
https://doi.org/10.3390/cancers12040905
FAQ
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What makes Eterneon-Red 645 Azide different from Cy5?
It offers improved water solubility and compatibility with aqueous click reactions, making it more versatile for biological applications.
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Can I use this dye for live cell imaging?
Yes, its non-toxic nature and bright fluorescence make it suitable for live cell tracking and imaging.
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Is it compatible with EdU assays?
Absolutely. It is a key reagent in EdU-based DNA replication assays, enabling precise detection of proliferating cells.
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What is the recommended concentration for labeling?
For a 10 mM solution, add 159 μL to 1 mg or 799 μL to 5 mg of dye.
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Are there enhanced variants available?
Yes, baseclick also offers Eterneon-Red 645-Picolyl Azide and AZDye 647 Azide Plus, featuring picolyl-azide and triazol-azide linkers for faster reaction kinetics and lower copper requirements.