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Product Name:
TTT mRNA prep Kit - 555

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For research use only!

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The TTT mRNA prep Kit contains reagents to perform 10 transcription reactions (50 µL each) and 10 independent labeling reactions.

Introduction and product description:
Baseclick’s TTT mRNA prep Kit has been developed for the production of labeled mRNA by in vitro transcription (IVT) from bacteriophage T7 RNA polymerase promoter templates. The kit utilizes a proprietary nucleotide mixture to label the mRNA with alkynes. After transcription these alkynes can be reacted with azido-derivates of fluorescent dyes, haptenes and other labels in a highly selective fashion under benign click reaction conditions. Fluorescently labeled mRNA can be used to detect localization of the mRNA inside of cells after transfection. In order to control mRNA production, click labeling, transfection and protein expression, the kit provides a control DNA template that codes for an eGFP mRNA.

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